peptide reconstitution calculator

calculate the exact concentration and syringe draw volume for any peptide. free, instant, no signup required.

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reconstitution basics

everything you need to know before mixing your first vial

reconstitution is the process of dissolving a freeze-dried (lyophilized) peptide powder back into a liquid solution so it can be measured and administered. most research peptides ship as a dry powder in a sealed vial and must be reconstituted before use.

lyophilization (freeze-drying) is a preservation method where water is removed from a frozen peptide solution under vacuum. the result is a stable, dry powder that can be stored for months without degrading. this is why most peptides arrive as a white or off-white powder or puck at the bottom of the vial.

peptides degrade rapidly in liquid form -- heat, light, and bacteria break them down. shipping and storing them as a dry powder preserves their potency. reconstitution converts them back into a measurable liquid right before use, ensuring you get the full dose with minimal degradation.

bacteriostatic water (BAC water) is sterile water that contains 0.9% benzyl alcohol as a preservative. the benzyl alcohol inhibits bacterial growth, which means a reconstituted vial can be stored and drawn from multiple times over days or weeks without contamination. it is the standard solvent for peptide reconstitution.

sterile water for injection can dissolve the peptide, but it lacks preservatives. without the benzyl alcohol found in bacteriostatic water, bacteria can grow in the solution after the first puncture. if you use sterile water, the entire vial should ideally be used in one session. for multi-dose vials, bacteriostatic water is strongly preferred.

draw bacteriostatic water into a syringe, then inject it slowly down the inside wall of the peptide vial -- do not spray directly onto the powder. let it slide down gently. once the water is added, swirl the vial slowly until the powder fully dissolves. never shake the vial, as this can damage the peptide structure.

frequently asked questions

there is no single correct amount. more water means a lower concentration and larger injection volumes. less water means higher concentration and smaller volumes. common choices are 1-2 mL for most peptide vials. use the calculator above to see how your choice affects the final dose.

on a standard U-100 insulin syringe, 100 units equals 1 mL. so 10 units = 0.1 mL, 5 units = 0.05 mL, and so on. this calculator converts your dose into insulin syringe units automatically.

store reconstituted peptides refrigerated at 2-8 degrees C (standard refrigerator temperature). most reconstituted peptides remain stable for 2-4 weeks when mixed with bacteriostatic water. do not freeze reconstituted peptides, and keep them away from light.

no. the total amount of peptide in the vial stays the same regardless of how much water you add. more water just means you need to draw a larger volume to get the same dose. less water means a smaller draw volume. the calculator handles this math for you.

no. this calculator is an educational tool that performs unit conversion math. it does not recommend dosages or peptides. always consult a qualified healthcare professional before using any peptide product.